Biochemical ReagentsTop Productsα1-Antitrypsin Assay Kit

α1-Antitrypsin Assay Kit

Abbreviation: AATMethod: Immunoturbidimetric Assay

Product Overview
  • Ready-to-use liquid two-reagent formulation, supplied in dedicated packaging and compatible with a wide range of automated clinical chemistry analyzers.
  • Measurement method: Two-point endpoint assay with a primary wavelength of 340 nm.
  • Wide linear range: Excellent linearity from 0.20–5.00 g/L (r ≥ 0.990).
  • Excellent stability: Stable for 12 months when stored at 2–8°C protected from light. Once opened, the reagents remain stable for up to 1 month at 2–8°C.
  • Sample compatibility: Suitable for serum and EDTA plasma.
  • Strong anti-interference performance: Effectively minimizes interference from lipemia, hemolysis, bilirubin, vitamin C (ascorbic acid), and other nonspecific substances.
  • Clinical Background

    This assay is intended for the in vitro quantitative determination of α1-antitrypsin (AAT) in human serum or plasma as an aid in clinical diagnosis.

    Serum AAT measurement is widely used in the evaluation of inflammatory conditions, liver disease, and protein-losing disorders.

    Elevated AAT concentrations are commonly observed in acute and chronic inflammatory conditions, including infections and rheumatoid diseases, as well as in trauma and tissue necrosis. Decreased AAT levels are frequently associated with severe liver injury, nephrotic syndrome, malnutrition, and other conditions affecting AAT synthesis or excessive protein loss.
    α1-Antitrypsin is a 54 kDa glycoprotein synthesized primarily by the liver. As the major serine protease inhibitor in human serum, it inhibits a variety of proteolytic enzymes, including neutrophil elastase, thrombin, urokinase, and other proteases. AAT also functions as an acute-phase protein. During inflammatory responses, increased vascular permeability allows AAT to diffuse into the interstitial fluid, where elevated local concentrations help limit protease-mediated tissue injury and contribute to the regulation of acute inflammation.

  • Assay Principle

    α1-Antitrypsin present in the sample specifically reacts with polyclonal anti-human α1-antitrypsin antibodies contained in the reagent to form insoluble immune complexes, resulting in increased turbidity. The degree of turbidity is directly proportional to the α1-antitrypsin concentration in the sample.
    By measuring the turbidity at 340 nm and applying a multipoint calibration curve, the α1-antitrypsin concentration in the serum or plasma specimen can be accurately quantified.

  • Available Packaging

    R1:1×15ml,R2:1×5ml   R1:2×75ml,R2:1×50ml   R1:1×30ml,R2:1×10ml   R1:2×96ml,R2:1×64ml  Siemens Analyzer Configuration：480 tests/kit(R1:1×45ml,R2:1×15ml)

    R1:1×45ml,R2:1×15ml   R1:3×15ml,R2:1×15ml   R1:1×60ml,R2:1×20ml   R1:4×30ml,R2:4×10ml  Calibrator：1×1ml

    R1:1×75ml,R2:1×25ml   R1:4×60ml,R2:4×20ml   R1:2×30ml,R2:2×10ml   R1:6×15ml,R2:6×5ml   Quality Control：2×0.5ml

    R1:2×45ml,R2:2×15ml   R1:6×30ml,R2:6×10ml   R1:2×60ml,R2:2×20ml   R1:6×60ml,R2:6×20ml