Biochemical ReagentsTop ProductsCreatine Kinase-MB Mass Assay Kit

Creatine Kinase-MB Mass Assay Kit

Abbreviation: CKMB massMethod: Latex-Enhanced Immunoturbidimetric Assay

Product Overview
  • Ready-to-use liquid two-reagent formulation, supplied in dedicated packaging and compatible with a wide range of automated clinical chemistry analyzers.
  • Measurement method: Two-point endpoint assay with a primary wavelength of 600 nm.
  • Wide linear range: Excellent linearity from 3.00–120.0 ng/mL (r ≥ 0.990).
  • Excellent stability: Stable for 12 months when stored at 2–8°C protected from light. Once opened, the reagents remain stable for up to 1 month at 2–8°C.
  • Sample compatibility: Suitable for serum and EDTA plasma.
  • Strong anti-interference performance: Effectively minimizes interference from lipemia, hemolysis, bilirubin, vitamin C (ascorbic acid), and other nonspecific substances.
  • Clinical Background

    This assay is intended for the in vitro quantitative determination of creatine kinase-MB (CK-MB) mass in human serum or plasma as an aid in clinical diagnosis.

    Serum CK-MB is an established cardiac biomarker widely used in the diagnosis of acute myocardial infarction (AMI) and in the assessment of myocardial infarct size.

    CK-MB is a cardiac-specific isoenzyme released into the circulation following myocardial injury. Serum CK-MB concentrations begin to rise in the early stage of acute myocardial infarction and continue to increase as myocardial damage progresses. Serial measurement of CK-MB can assist clinicians in evaluating the occurrence and progression of myocardial infarction, as well as monitoring the effectiveness of therapeutic interventions.

  • Assay Principle

    The assay employs proprietary technology to directionally immobilize CK-MB-specific antibodies onto latex microparticles. CK-MB present in the sample specifically binds to the corresponding antibodies in the reagent, forming antigen-antibody immune complexes that produce an increase in turbidity. The latex particles significantly enhance the turbidity change, thereby improving assay sensitivity.
    The increase in turbidity is directly proportional to the CK-MB concentration in the sample. By comparing the measured turbidity with that of calibrated standards, the CK-MB concentration in the serum or plasma specimen can be quantitatively determined.

  • Available Packaging

    R1:1×15ml,R2:1×5ml   R1:2×75ml,R2:1×50ml   R1:1×30ml,R2:1×10ml   R1:2×96ml,R2:1×64ml  Siemens Analyzer Configuration：480 tests/kit(R1:1×45ml,R2:1×15ml)

    R1:1×45ml,R2:1×15ml   R1:3×15ml,R2:1×15ml   R1:1×60ml,R2:1×20ml   R1:4×30ml,R2:4×10ml  Calibrator：6×0.5ml

    R1:1×75ml,R2:1×25ml   R1:4×60ml,R2:4×20ml   R1:2×30ml,R2:2×10ml   R1:6×15ml,R2:6×5ml   Quality Control：2×0.5ml

    R1:2×45ml,R2:2×15ml   R1:6×30ml,R2:6×10ml   R1:2×60ml,R2:2×20ml   R1:6×60ml,R2:6×20ml