Biochemical ReagentsTop ProductsGlutathione Reductase Assay Kit

Glutathione Reductase Assay Kit

Abbreviation: GRMethod: Glutathione Substrate Method

Product Overview
  • Ready-to-use liquid two-reagent formulation, supplied in dedicated packaging and compatible with a wide range of automated clinical chemistry analyzers.
  • Measurement method: Kinetic assay with a primary wavelength of 340 nm.
  • Wide linear range: Excellent linearity from 10.00–300.00 U/L (r ≥ 0.990).
  • Excellent stability: Stable for 12 months when stored at 2–8°C protected from light. Once opened, the reagents remain stable for up to 1 month at 2–8°C.
  • Sample compatibility: Suitable for serum and EDTA plasma.
  • Strong anti-interference performance: Effectively minimizes interference from lipemia, hemolysis, bilirubin, vitamin C (ascorbic acid), and other nonspecific substances.
  • Clinical Background

    This assay is intended for the in vitro quantitative determination of glutathione reductase (GR) activity in human serum or plasma as an aid in clinical diagnosis.

    Glutathione reductase is an important biomarker for the early detection of acute liver injury.

    During the early stage of acute hepatitis, serum GR demonstrates exceptionally high diagnostic sensitivity and serves as a valuable indicator for the early identification of hepatocellular injury. In patients with acute hepatitis, serum GR activity rises and reaches its peak earlier than conventional liver enzymes such as ALT and GGT, making it a preferred biomarker for the assessment of early liver damage.
    Serum GR activity is also useful for identifying subclinical drug-induced liver injury (DILI), thereby improving the clinical detection rate of DILI. Unlike ALT and AST, which are released following hepatocellular injury, GR is more closely associated with disruption of hepatocyte and mitochondrial membrane integrity. As a result, GR provides complementary information during the progression from early cellular injury to irreversible cell damage, facilitating earlier diagnosis and timely clinical intervention in liver disease.

  • Assay Principle

    Glutathione reductase (GR) in the sample catalyzes the reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH) in the presence of nicotinamide adenine dinucleotide phosphate, reduced form (NADPH), while NADPH is simultaneously oxidized to NADP⁺.
    NADPH exhibits a characteristic absorbance peak at 340 nm. The rate of decrease in absorbance is directly proportional to the GR activity present in the sample. By continuously monitoring the decline in NADPH absorbance at 340 nm, the activity of glutathione reductase can be accurately determined.

  • Available Packaging

    R1:1×20ml,R2:1×5ml   R1:2×72ml,R2:1×36ml   R1:1×80ml,R2:1×20ml   R1:6×20ml,R2:6×5ml    Siemens Analyzer Configuration：400 tests/kit(R1:1×40ml,R2:1×10ml)

    R1:1×60ml,R2:1×15ml   R1:4×80ml,R2:4×20ml   R1:2×60ml,R2:2×15ml   R1:6×80ml,R2:6×20ml  Calibrator：1×0.5ml

    R1:2×40ml,R2:2×10ml   R1:6×40ml,R2:6×10ml   R1:4×40ml,R2:4×10ml                                        Quality Control：2×0.5ml

    R1:2×80ml,R2:2×20ml   R1:1×40ml,R2:1×10ml   R1:2×96ml,R2:1×48ml