Biochemical ReagentsTop ProductsHaptoglobin Assay Kit

Haptoglobin Assay Kit

Abbreviation: HPTMethod: Immunoturbidimetric Assay

Product Overview
  • Ready-to-use liquid two-reagent formulation, supplied in dedicated packaging and compatible with a wide range of automated clinical chemistry analyzers.
  • Measurement method: Two-point endpoint assay with a primary wavelength of 340 nm.
  • Wide linear range: Excellent linearity from 0.20–3.00 g/L (r ≥ 0.990).
  • Excellent stability: Stable for 12 months when stored at 2–8°C protected from light. Once opened, the reagents remain stable for up to 1 month at 2–8°C.
  • Sample compatibility: Suitable for serum and EDTA plasma.
  • Strong anti-interference performance: Effectively minimizes interference from lipemia, hemolysis, bilirubin, vitamin C (ascorbic acid), and other nonspecific substances.
  • Clinical Background

    This assay is intended for the in vitro quantitative determination of haptoglobin (HPT) in human serum or plasma as an aid in clinical diagnosis.

    Haptoglobin is primarily used as an important biomarker for the assessment of intravascular hemolytic disorders.

    Also known as hemoglobin-binding protein, haptoglobin is an acute-phase glycoprotein synthesized by hepatocytes. It consists of two α (alpha) chains and two β (beta) chains. Haptoglobin binds free hemoglobin released during hemolysis and transports the resulting complex to the liver for degradation.

    During intravascular hemolysis, haptoglobin is consumed much more rapidly than it can be synthesized by the liver. Consequently, any condition associated with increased erythrocyte destruction and elevated free hemoglobin leads to a reduction in serum haptoglobin levels. Serum haptoglobin concentrations are markedly decreased in hemolytic anemia and liver cell injury, while they are significantly elevated in inflammatory conditions, trauma, and coronary artery disease as part of the acute-phase response.
    Assay Principle

    Haptoglobin present in the sample specifically reacts with anti-human haptoglobin antibodies contained in the reagent to form insoluble immune complexes, resulting in increased turbidity. The degree of turbidity is directly proportional to the haptoglobin concentration in the sample. By measuring the change in absorbance at 340 nm and applying a multipoint calibration curve, the haptoglobin concentration can be accurately quantified.

  • Available Packaging

    R1:1×20ml,R2:1×5ml   R1:2×72ml,R2:1×36ml   R1:1×80ml,R2:1×20ml   R1:6×20ml,R2:6×5ml  Siemens Analyzer Configuration：400 tests/kit(R1:1×40ml,R2:1×10ml)

    R1:1×60ml,R2:1×15ml   R1:4×80ml,R2:4×20ml   R1:2×60ml,R2:2×15ml   R1:6×80ml,R2:6×20ml   Calibrator：1×1ml

    R1:2×40ml,R2:2×10ml   R1:6×40ml,R2:6×10ml   R1:4×40ml,R2:4×10ml                                      Quality Control：2×0.5ml

    R1:2×80ml,R2:2×20ml   R1:1×40ml,R2:1×10ml   R1:2×96ml,R2:1×48ml