Biochemical ReagentsTop ProductsType IV Collagen Assay Kit

Type IV Collagen Assay Kit

Abbreviation: IV COLMethod: Latex-Enhanced Immunoturbidimetric Assay

Product Overview
  • Ready-to-use liquid two-reagent formulation, supplied in dedicated packaging and compatible with a wide range of automated clinical chemistry analyzers.
  • Measurement method: Two-point endpoint assay with a primary wavelength of 570 nm.
  • Wide linear range: Excellent linearity from 20.00–600.00 ng/mL (r ≥ 0.990).
  • Excellent stability: Stable for 12 months when stored at 2–8°C protected from light. Once opened, the reagents remain stable for up to 1 month at 2–8°C.
  • Sample compatibility: Suitable for serum and EDTA plasma.
  • Strong anti-interference performance: Effectively minimizes interference from lipemia, hemolysis, bilirubin, vitamin C (ascorbic acid), and other nonspecific substances.


  • Clinical Background

    This assay is intended for the in vitro quantitative determination of Type IV Collagen in human serum or plasma as an aid in clinical diagnosis.

    Type IV Collagen is an important biomarker for evaluating liver fibrosis and cirrhosis. Serum Type IV Collagen concentrations correlate closely with the degree of hepatic fibrosis and can therefore assist clinicians in assessing the severity of liver cirrhosis.


    In acute hepatitis, although extensive hepatocyte damage occurs, there is little connective tissue proliferation. Consequently, serum Type IV Collagen levels generally do not differ significantly from those of healthy individuals.
    In patients with chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma, serum Type IV Collagen levels are significantly elevated. The degree of elevation generally follows the order: primary liver cancer > liver cirrhosis > chronic active hepatitis > chronic persistent hepatitis > acute viral hepatitis.
  • Assay Principle

    Type IV Collagen-specific antibodies are directionally immobilized onto latex microparticles. During the reaction, Type IV Collagen present in the sample specifically binds to the corresponding antibodies in the reagent, forming antigen-antibody immune complexes in the liquid phase. This reaction produces an increase in turbidity, which is significantly amplified by the latex particles to improve assay sensitivity.
    The increase in turbidity is directly proportional to the concentration of Type IV Collagen in the sample. By comparing the measured turbidity with that of a calibrated standard, the concentration of Type IV Collagen in the serum or plasma specimen can be quantitatively determined.

  • Available Packaging

    R1:1×15ml,R2:1×5ml   R1:2×75ml,R2:1×50ml   R1:1×30ml,R2:1×10ml   R1:2×96ml,R2:1×64ml  Siemens Analyzer Configuration：480 tests/kit(R1:1×45ml,R2:1×15ml)

    R1:1×45ml,R2:1×15ml   R1:3×15ml,R2:1×15ml   R1:1×60ml,R2:1×20ml   R1:4×30ml,R2:4×10ml  Calibrator：6×0.5ml

    R1:1×75ml,R2:1×25ml   R1:4×60ml,R2:4×20ml   R1:2×30ml,R2:2×10ml   R1:6×15ml,R2:6×5ml   Quality Control：2×0.5ml

    R1:2×45ml,R2:2×15ml   R1:6×30ml,R2:6×10ml   R1:2×60ml,R2:2×20ml   R1:6×60ml,R2:6×20ml