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Excessive free radicals in the body will damage proteins, cell membranes, and promote cell tissue DNA mutations, thereby inducing or accelerating the production and deterioration of human diseases. In many cases, such as radiation injury, inflammation and emergency response, tumor lesion, reperfusion injury, aging, etc., most of them are accompanied by abnormal increase of free radicals. Superoxide dismutase (sod) is an important scavenger of free radical superoxide anion in vivo.
In this method, catechol self-oxidation method is adopted. Catechol in alkaline working solution will produce self-oxidation chromogenic reaction. During the process of self-oxidation, superoxide anion radical (02-) will be continuously released. While SOD activity decreases, disproportionation reaction is inhibited and free radical (O2-) concentration increases, thus resisting the autoxidation of catechol. Its reaction intensity is weak, which is, the chromogenic reaction intensity of catechol autoxidation in the reaction is positively correlated with the activity of SOD.