Serum CG levels were elevated in acute hepatitis, chronic active hepatitis, cirrhosis, and chronic prolonged hepatitis. CG can be used as a valuable indicator for the identification of chronic active hepatitis and chronic prolonged hepatitis, especially in labor.

Free glycolic acid in the sample competitively binds to anti-glycolic acid-specific antibody sites with the glucose-6-phosphate dehydrogenase - glycolic acid coupling. The more free cholic acid in the sample, the more antibody sites competing for binding, and the more enzyme conjugates released by the antibody. The free glycinase conjugated compound catalyzes the conversion of the beta-nicotinamide adenine dinucleotide oxidation type (NAD+) to the beta-nicotinamide adenine dinucleotide reduction (NADH). The concentration of cholic acid in the sample is proportional to the amount of NADH produced. The content of glyceric acid can be calculated by the change of the absorption value of 340nm.